Thank you to our Platinum Sponsor

WEDNESDAY 26 NOVEMBER 2025
1600 – 1800 Registration Level 3, Foyer 1
1800 – 1900 Welcome ReceptionSponsored by Level 3, Bellevue Ballroom 1
THURSDAY 27 NOVEMBER 2025
0800 – 1730 Registration Level 3, Foyer 1
0815 – 1545 Exhibition Opens Bellevue Ballroom 1
0815 – 1715 Speaker Preparation Room Opens Meeting Room 12, Level 2
0845 – 1000 Session 1 – Plenary
Chairs: Dr Matt Linden & Dr Henry Hui
Bellevue Ballroom 2
0845 – 0850 Welcome to Country
Freda Ogilvie, Whadjuk / Balladong Noongar Elder
0850 – 0900 Opening Speech and Announcements
Dr Matt Linden, Conference Chair
0900 – 1000 Convergence in Cytometry: Rainbows and Reasoning in the Pursuit of AML Residual Disease
Dr Paul Wallace, Roswell Park Cancer Centre
1000 – 1030 Morning Tea Sponsored by Bellevue Ballroom 1
1030 – 1215 Session 2 – Plenary
Chairs: Dr Chris Tjiam & Dr Andrew Lim
Bellevue Ballroom 2
1030 – 1130 New metrics to achieve high-quality data in the era of 50-color+ flow cytometry
Dr Florian Mair, ETH Zurich
1130 – 1215 Platinum Sponsor Presentation- Diving Deep: Profiling Exhausted T Cells in the Tumor Microenvironment Using Full Spectrum Flow Cytometry
Karen Teng, Merck Sharp & Dohme (MSD) 
1215 – 1315 Lunch Sponsored by Bellevue Ballroom 1
1315 – 1515 Session 3 – Clinical Breakout (Haematology)
Chairs: Kristy Montana & Fiona Robins
Meeting Room 2
Session 4 – Research Breakout (High Dimensional Panels)
Chairs: Kate Pilkington & Dr Florian Mair
Bellevue Ballroom 2
Session 5 – Research Breakout (Outside the box applications)
Chairs: Dr Henry Hui & Dr Jochen Behrend
Meeting Room 1
1315 – 1345 Minimal Residual Disease in Acute Lymphoblastic Leukemia testing
Dr Ben Hedley, London Health Sciences Center
High-resolution profiling of vaccine-induced B cell responses with full-spectrum flow cytometry
Dr Christian Tjiam, The Kids Research Institute Australia
Targeting microbes using spectral cytometry
Dr Allison McInnes, The Centre for Microbiome Research
1345 – 1405 RHH Flow: A Journey of Validation (2 DxFLEX and a CellMek SPS)
Emily Hinds, Royal Hobart Hospital
Delineating the alloresponse in solid organ transplantation with flow cytometry
Dr Amy Prosser, University of Western Australia
Spectral cytometry empowers a no-stain assay to study global-warming inducing methanogens in their natural milieu; a useful tool advancing anti-methanogen vaccine design
Joanna Roberts, Flowjoanna Tāpui
1405 – 1415 Fluorescence-activated protoplast sorting for crop improvement
Dr Sareena Sahab, Department of Energy, Environment and Climate Action
1415 – 1425 Spectral flow cytometry in clinical haematology
Associate Professor Elizabeth Tegg, NSW Health Pathology
Spatial profiling of the tumour microenvironment in hepatocellular carcinoma
Dr Felix Marsh-Wakefield, Centenary Institute
1425 – 1445 Imaging Flow Cytometric Detection of IGH Translocations in Multiple Myeloma
Sarah Clarke, University of Western Australia
1445 – 1515 Verification of the dual TRBC1 and TRBC2 testing for assessment of T-cell clonality at the Royal Prince Alfred Hospital Haematology laboratory
Dr Ji Do, Royal Prince Alfred Hospital
Improved Single-Cell Multiomics through Optimised Sequence Utilisation
Dr Wenyan Li, Children’s Cancer Institute Australia
Leveraging cytometry methods in a cohort of transgender young people to detect hormone-mediated changes in immune function
Alice White, The Kids Research Institute Australia
1515 – 1545 Afternoon Tea Sponsored by Bellevue Ballroom 1
1545 – 1715 Session 6 – Clinical Breakout (Haem Onc)
Chairs: Tania Jobey & Laura Hill
Meeting Room 2
Session 7 – Research Breakout (Non-Tumour Immunology)
Chairs: Dr Paula Niewold & Dr Connie Jackaman
Bellevue Ballroom 2
Session 8 – SRL Breakout
Chairs: Dr Catherine Rinaldi & Avril Aspland
Meeting Room 1
1545 – 1605 Advances in diagnostics and treatment for infants with acute lymphoblastic leukaemia
Professor Rishi Kotecha, Perth Children’s Hospital
High-Dimensional Spectral Flow Cytometry Demonstrates Impaired B Cell Responses to Childhood Vaccination: Preliminary Findings from the CHIP study (Childhood Health and Immunity Post-burn Injury)
Dr Lucy Barrett, University of Western Australia
Integration of New Technologies into Shared Service Facilities: Image-based Cell Sorting
Alan Saluk, The Scripps Research Institute
1605 – 1625 The Proportion of Circulating Tumor Plasma Cells out of Total Circulating Plasma Cells is a Good Prognostic Indicator for Newly Diagnosed Patients of Multiple Myeloma
Dr Man Updesh Singh Sachdeva, Postgraduate Institute of Medical Education and Research (PGIMER)
Full-spectrum flow cytometry reveals mucosal tissue-based immunity induced by a live bacterial intranasal vaccine
Jack Pepper, The Kids Research Institute Australia
Darkness be my Friend: When Reduced Laser Power and Clever Chemistry can Improve Signal Resolution
Steven Lim, Francis Crick Institute
1625 – 1645 Role of TRBC1 and TRBC2 in assessment of T cell clonality
Professor William Sewell, Garvan Institute
Influence of Baseline Immune Composition on Neutralising Antibody and T Cell Responses to SARS-CoV-2 Vaccination
Sarah Baird, The University of Sydney
Building Integrated Precision Medicine Pipelines Across Shared Resource Laboratories via the PrecisionGo Initiative
Dr Maggie Wang, Westmead Institute for Medical Research
1645 – 1715 Prognostic Value of Dynamic Minimal Residual Disease Monitoring for Adolescent and Adult T-lymphoblastic Leukemia /lymphoma
Hongyan Liao, West China Hospital, Sichuan University
Spectral Cytometry Phenotyping of Dendritic Cells in Autoimmunity
Nadia Roberts, John Curtin School of Medical Research
The Critical Aspects of Aerosol Containment Testing in Flow Cytometry – What are the essential requirements for safety?
Avrill Aspland, The University of Sydney
1715 – 1800 Session 9 – Keynote
Chairs: Dr Matt Linden & Rob Salomon
The Role of Interdisciplinary Research in Advancing Clinical Excellence
Professor Fiona Wood AO, FRCS, FRACS, Fiona Wood Foundation
Bellevue Ballroom 2
1800 – 1900 Poster viewing with refreshments Bellevue Ballroom 1
FRIDAY 28 NOVEMBER 2025
0800 – 0845 Commercial Breakfast & Learn Sponsored by
0815 – 1730 Registration PCEC Level 3 Foyer 1
0830 – 1620 Exhibition Opens Bellevue Ballroom 1
0830 – 1730 Speaker Preparation Room Opens Meeting Room 12, Level 2
0900 – 1000 Session 10 – Plenary
Chairs: Dr Pat Metharom & Eva Orlowski-Oliver
0900 – 1000 The Technician Commitment: Transforming Technical Careers through Culture Change
Dr Kelly Vere MBE, UK Institute for Technical Skills & Strategy and University of Nottingham
Bellevue Ballroom 2
1000 – 1030 Morning Tea Sponsored by Bellevue Ballroom 1
1030 – 1250 Session 11 – Plenary
Chairs: Dr Maggie Wang & Dr Jamie Rodgers
Bellevue Ballroom 2
1030 – 1130 Combatting the Emerging Threat of Antimicrobial Resistance with AI-Enhanced Flow Cytometry
Dr Kieran Mulroney, Cytophenix
1130 – 1150 Gold Sponsor Presentation – The Fastest Path To Discovery: Combining Acoustic Focusing With Spectral Cytometry
Dr Brandon Trent, Thermo Fisher Scientific
1150 – 1250 “Immuno-flowFISH”: Imaging Flow Cytometry to Detect Chromosomes in Haematological Malignancies
Professor Wendy Erber, University of Western Australia
1250 – 1350 Lunch Sponsored by Bellevue Ballroom 1
1350 – 1550 Invited Speakers
1350 – 1550 Session 12 – Clinical Breakout (Infection and Immunity)
Chairs: Monica Kemp & Dr Kathryn Hally
Meeting Room 2
Session 13 – SRL Breakout
Chairs: Dr Pat Metharom & Rob Salomon
Bellevue Ballroom 2
Session 14 – Innovation Breakout
Chairs: Dr Jessica Houston & Dr Jingjing Zhao
Meeting Room 1
1350 – 1400 Inborn Errors of Immunity: What Can They Tell Us About Common Allergic Diseases?
Professor Cindy Ma, Garvan Institute of Medical Research
Introduction – Technician Commitment and ACRI in Aus/NZ
Rob Salomon, ACS president
Detection of High-Risk Chromosomal Abnormalities in the Circulation in Myelofibrosis using Imaging Flow Cytometry
Ruby Hamilton, The University of Western Australia
14:00 – 14:10 Projecting Outwards, Reflecting Inwards: How Technician-Led Change Shaped My Path from Flow to Faculty
Professor Andy Filby, Newcastle University Biosciences Institute (NUBI)
14:10 – 14:20 High-Dimensional Immunophenotyping of Circulating Neutrophils Using a Novel 17-Marker Spectral Flow Cytometry Panel.
Dr Ceridwyn Jones, University of Otago
1420 – 1430 Innovative Clinical Flow Cytometry Panels in the Investigation of Primary Immunodeficiency
Dr Sharon Choo, The Royal Children’s Hospital Melbourne
1430 – 1450 Panel Discussion

The importance of research technicians. Their current status in Academia and Research Institutes: what can we do to improve them

Panellists:

  • Dr Kelly Vere MBE – University of Nottingham
  • Professor Andrew Filby –  Newcastle University
  • Professor Michele Wilson – Curtin University Associate Provost
  • Dr Scott Fitzgerald – National Tertiary Education Union (WA Division)
  • Adj Professor Sach Jayasinghe – director of ACRI (Academy for Collaborative Research Infrastructure)
Rethinking Diagnostics in Myelodysplastic Syndrome and Unexplained Cytopenia: Employing Morphometric Evaluation of Dysplasia by Imaging Flow Cytometry
Carina Rosenberg, Aarhus University Hospital
1450 – 1510 Design and Findings from the Plastic Exposure Reduction Transforms Health (PERTH) Trial
Professor Michaela Lucas, University of Western Australia, NHMS, CAHS
Detection of Adverse Cytogenetics in Acute Myeloid Leukaemia by Imaging Flow Cytometry using “Immuno-flowFISH”
Naomi Damstra, The University of Western Australia
1510 – 1520 Defining Surface Receptor Phenotypes of Airway Mucosal B Cells using Massively Parallel Flow Cytometry
Edison Foo, The Kids Research Institute Australia
1520 – 1550 Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel
Dr Ainsley Davies, The Australian National University
Decade of the Cytometry Core: Balancing Strategic planning amid the day-to-day challenges of a growing facility
Dr Randall Grose, SAHMRI
1550 – 1620 Afternoon Tea Sponsored by Bellevue Ballroom 1
1620 – 1740 Session 15 – Clinical Breakout (Infection & Immunity)
Chairs: Christian Tjiam & Dr John Zaunders
Meeting Room 2
Session 16 – Innovation Breakout
Chair: Dr Wenyan Li, Dr Jessica Houston
Bellevue Ballroom 2
Session 17 – Research Breakout (Tumour Immunology)
Chairs: Dr Bree Foley & Dr Sonia Fernandez
Meeting Room 1
1620 – 1640 Basophil Activation Test: a Future Alternative to Oral Food Challenge for Pediatric Peanut Allergy?
Dr Jochen Behrends, Research Center Borstel
Lifetime Measurement by Time-Correlated Multi-Photon Counting (TCMPC) in Quantum Cytometry
Masanobu Yamamoto, MIftek Corporation
Identifying Immune-Related Biomarkers of Chemoradiotherapy Response in Rectal Cancer using Flow and Tissue Cytometry
Dr Melanie Mccoy, St John of God Subiaco Hospital
1640 – 1700 High Parameter Spectral Flow Cytometry Panels: Adapting from Research Use Only to a Clinical Trial Setting in an IS017025-Acccredited Laboratory
Dr Elizabeth McDonald, QIMR Berghofer
Structured Illuminations in Biomedical Microscopy with Diffractive Optics
Dr Jingjing Zhao, Huazhong University of Science and Technology
Identifying Predictors of Colorectal Cancer Metastasis using Multiplex Immunofluoresence and Digital Image Analysis
Tracey Lee-Pullen, St John of God Subiaco Hospital
1700 – 1720 Functional Evaluation of Rare Plasmacytoid Dendritic Cells in Children with Asthma
Dr Jonatan Leffler, The Kids Research Institute Australia
Machine Learning-Based Biomarker Development for Immunotherapy Response Prediction in Resectable Hepatocellular Carcinoma
Andrea (Yu-Fen) Wang, AHEAD Medicine Corporation
Identification of Cancer-Associated Fibroblast Populations that Affect CD8 T Cell Infiltration into Tumours of Colorectal Cancer Patients
Dr Roslyn Kemp, University of Otago
1720 – 1740 Understanding the Impact of Childhood Burn Injury on Immunity: From Conventional to Spectral Flow Cytometry
Dr Blair Johnson, Fiona Wood Foundation
Spectral Flow Cytometry Revisited: Information-Theoretic Principles, Optimal Design, and Why Having More Channels is Not Always Better
Dr Bartek Rajwa, Purdue University
Comprehensive Cord Blood NK Cell Subsets Characterisation and Ligand-Receptor Mapping to Optimise to NK cell Immunotherapy in Paediatric AML
Bhumika Tirthani, Centre For Cancer Biology, University of South Australia
1820 Meet at the Coach Bay for transfers to Optus Stadium. Coach Bay is located at the ground level of PCEC
1900 – 2200 Conference DinnerSponsored by Riverview Room, Optus Stadium
SATURDAY 29 NOVEMBER 2025
0830 – 1430 Registration Level 3, Foyer 1
0830 – 1300 Exhibition Opens Bellevue Ballroom 1
0830 – 1500 Speaker Preparation Room Opens Meeting Room 12, Level 2
0900 – 1000 Session 18 – Plenary
Chairs: Prof Bill Sewell & Andrea (Yu-Fen) Wang
Bellevue Ballroom 2
0900 – 1000 Diagnosis and evaluation of acute leukaemia of ambiguous lineage by flow cytometry
Dr Franklin “Buddy” Fuda, UT Southwestern Medical Center
1000 – 1030 Morning Tea Bellevue Ballroom 1
1030 – 1150 Session 19 – Plenary
Chairs: Pradeep Kumar & Dr Laila Abudulai
Bellevue Ballroom 2
1030 – 1130 A Tale of Two Flow Cells and Four Viruses
Dr Vainav Patel, NIRRCH (ICMR)
1130 – 1150 Gold Sponsor Presentation: Tips for accurate high-dimensional analysis and sorting with Spectral Flow Cytometry
Makoto Ishihara
1150 – 1250 Lunch Bellevue Ballroom 1
1250 – 1430 Parallel Sessions – Invited Speakers
1250 – 1430 Session 20 – Breakout (Cytometry Education)
Chairs: Dr Matt Linden & Dr Paresh Jain
Meeting Room 2
Session 21 – Breakout (Partnerships)
Chairs: Joanna Roberts &
Dr Catherine Rinaldi
Bellevue Ballroom 2
Session 22 – Breakout (Mass & Imaging Cytometry)
Chairs: A/Prof Helen McGuire &
Dr Felix Marsh-Wakefield
Meeting Room 1
Session 23 – Breakout (Applications)
Chairs: Dr Givanna Putri &
Dr Chris Tjiam
Meeting Room 3
1250 – 1300 TCS and Cytometry Education in India
Dr Vainav Patel, NIRRCH (ICMR)
Rapid Characterisation and Quantification of Toxin Producing Cyanobacteria in Limnic Systems by Imaging Flow Cytometry
Dr Kathy Fuller & Luca Zappia, The University of Western Australia & Water Corporation of Western Australia
1300 – 1320 Mass Cytometry Ensures Deep Immune Profiling in Large-Scale Studies when Planned and Implemented Successfully
Thomas Hueneburg, Charles Perkins Centre / University of Sydney
Flow Cytometric Detection of BCR::ABL1 in T-lymphocytes in Chronic Myeloid Leukaemia
Daria Buic, The University of Western Australia
1320 – 1340 Flow Cytometry Education in Southeast Asia – The ISAC Live Education Delivery Group Experience
Dr Paul Hutchinson, National University of Singapore
Statistical Comparison of Clusters in 18-colour Flow Cytometry Reveals a Novel Expanded Subset of CMV-Specific CD4 T Cells
Professsor John Zaunders, St Vincent’s Hospital
An In Situ Quantitative Map of Mononuclear Phagocyte Interactions with HIV across Anogenital Mucosal Tissues Using High Parameter Spatial Techniques
Dr Thomas O’Neil, The Westmead Institute for Medical Research
Investigating Phenotypic and Functional Changes in Peanut-Specific B and T Lymphocytes throughout the Course of Peanut Oral Immunotherapy in Allergic Infants
Dr Abbey Figliomeni, The Kids Research Institute
1340 – 1350 Utilising High-Parameter Flow Cytometry and Imaging Mass Cytometry to Study Resident and Infiltrating Immune Cell Subsets in Herpes Keratitis Diseased Human Corneas
Dr Kirstie Bertram, Westmead Institute for Medical Research
Imaging Flow Cytometric Detection of CRLF2 rearrangement and TSLPR in Ph-like Acute Lymphoblastic Leukaemia
Andrea Aluner, Translational Cancer Pathology Laboratory, University of Western Australia
1350 – 1400 The Need for Clinical Flow Training in Regional Areas
Ailie Ross, James Cook University
A Partnership for the Use of Lifetime-Cytometry and the Study of Metabolic Factors that Affect Cancer Cell Resistance to Therapy
Dr Jessica Houston & Professor Kevin Houston, New Mexico State University
1400 – 1430 Novel Strategy to Enable Equity in High Parameter Immune Phenotyping Studies for Individuals from Remote Settings
Natalie Smith, University of Sydney
Harnessing the Immune Response to Vascular Surgery for Biomarker Discovery
Connor Davis, University of Otago
1430 – 1530 Session 24 – ACS AGM, Council Elections, Awards Ceremony and Conference Close
Chairs: Rob Salomon & Michael Thomson
Annual General Meeting of the Australasian Cytometry Society, including council elections, conference and travel awards, and closing remarks. All delegates are welcome to participate.
Bellevue Ballroom 2
SUNDAY 30 NOVEMBER 2025 – Workshops
Venue Harry Perkins Institute of Medical Research (North)
0900 – 0930 Arrival Coffee & Tea
0930 – 1230 What’s Special about Spatial? (Half Day) Spectral Panels (Half Day) Imaging Cytometry (Half Day) Clinical Flow (Half Day) SRL (Half Day)
G17 eSuite
M Block “green”
Chairs: Dr Thomas O’Neil & Dr Felix Marsh-Wakefield
McCusker Auditorium
QQ Block
Chair: Dr Chris Tjiam
G15 eSuite
M Block “red”
Chair: Dr Henry Hui
G24 Seminar Room
QQ Block
Chair: Kristy Montana
G19 eSuite
M Block “blue”
Chairs: Dr Pat Metharom & Dr Catherine Rinaldi
0930 – 1000 What’s Special about Spatial?
Session 1
Dr Thomas O’Neil
Dr Givanna Putri
Spectral Flow Cytometry Fundamentals
Kate Pilkington
Imaging Cytometry
Dr Henry Hui
Dr Kathy Fuller
Dr Paul Wallace

Dr Jochen Behrends
Dr Carina Rosenberg
Multiple Myeloma Minimal Residual Disease detection
Dr Ed Abadir
Era of Technical Specialists – Survey and Discussion
Dr Pat Metharom
Dr Catherine Rinaldi

Eva Orlowski-Oliver

ACS Technician Commitment Action Plan Update and Discussion
ACS TC Taskforce
1000 – 1030 Spectral Panel Design Task
Dr Florian Mair
1030 – 1100 SuperRCA, an innovative Mutation Detection technology: Bridging Molecular Biology and Flow Cytometry
Shankar Pattabhiraman
How to Grow your Flow SRLs
Michael Thomson
Professor Andy Filby & Alan Saluk

Strategies for Success in Operating a Shared Resource Flow Cytometry Facility
Alan Saluk
1100 – 1130
1130 – 1200 Spectral Panel Troubleshooting
Dr Ainsley Davies
RCPA
Haematology Immunophenotyping Update
Loriza Khan
ISAC SRL Recognition Program – An interactive dive into the program application, benefits and reluctancies
Eva Orlowski-Oliver
Jessica Lakshmi Prieto-Chávez
Edwin Lau
Matilda Mostrom
1200 – 1230
1230 – 1315 Lunch
1315 – 1615 Spatial Cytometry Masterclass: Public Data Repositories (Half Day) Low Flow & Working with the Weird (Half Day) Safety & Publishing (Half Day) Innovation & Optimization (Half Day)
G17 eSuite
M Block “green”
Chairs: Dr Thomas O’Neil & Dr Felix Marsh-Wakefield
G24 Seminar Room
QQ Block
Chairs: Dr Matt Linden & Dr Pat Metharom
McCusker Auditorium
QQ Block
Chair: Dr Catherine Rinaldi
G19 eSuite
M Block “blue”
Chairs: Dr Henry Hui & Dr Kathy Fuller
1315 – 1345 Upcycling Someone Else’s Data: Capitalising on Public Data Repositories  Session 2 
Dr Felix Marsh-Wakefield
Dr Paula Niewold
Low Flow: Selecting a limited flow cytometry panel where resources are constrained
Ailie Ross
Sample-Associated Biological Safety Assessment: A Scenario-Driven Workshop
Avrill Aspland
Beyond Panel Design:
Optimization Strategies for High-Quality, Reproducible Flow Cytometry Experiments
Dr Kathryn Hally
Kate Pilkington
1345 – 1415 Panel: Future of AI in Clinical Flow Cytometry
1415 – 1425 Welcome and live polling
Pat Metharom
Application of Cell Painting Techniques Using an Imaging Flow Cytometer
Dr Haley Pugsley
1425 – 1445 Illuminating the Invisible: Reliable Small Vesicle Detection with Fluorogenic, Membrane-Specific Conjugated Oligoelectrolytes
Bay Sie Lim
1445 – 1505 High-Purity Sorting of Specific Extracellular Vesicle Populations Using Fluorescence-Activated Cytometry
Jochen Behrends
1505 – 1515 Practical Considerations For An ACS Clinical Guideline in Platelet Flow Cytometry (*)
Matt Linden & Kathryn Hally
1515 – 1525 Join the mission: Advancing cytometry through publishing, reviewing, and editing
Dr Bartek Rajwa
Dr Jessica Houston
Laser Beam Shaping with Diffractive Optics for Enhanced Flow Cytometry
Jingjing Zhao
1525 – 1545 Spectral cytometry empowers a no-stain assay to study global-warming inducing methanogens in Rumens
Joanna Roberts
1545 – 1605 Sorting the human faecal microbiome
Allison McInnes
1605 Follow up discussion and close
Pat Metharom